SPECTROSCOPIC AND CHROMATOGRAPHIC ANALYSIS OF BIOACTIVE COMPOUNDS PRESENT IN MEDICINAL PLANT CAESALPINIA CRISTA
Aryavrat Singh, Monti Pal, Rishika Mishra, Jaswinder Mehta, Peenu Mahendra Joshi, Ruchi Acharya, Bhavna Sharma
ABSTRACT
The present study was undertaken to investigate the phytochemical composition and chromatographic profile of the aqueous extract of Caesalpinia crista, a medicinal plant widely used in traditional medicine. The aqueous extract was prepared and evaluated for extractive yield, preliminary phytochemical constituents, total phenolic content, total flavonoid content, and Thin Layer Chromatography (TLC) fingerprinting. The extract yielded 4.92% w/w and appeared as a black-colored residue. Preliminary phytochemical screening revealed the presence of glycosides, flavonoids, saponins, proteins, diterpenes, and phenolic compounds, while alkaloids, carbohydrates, tannins, and sterols were absent. Quantitative estimation showed total phenolic and total flavonoid contents of 0.895 mg/100 mg and 0.742 mg/100 mg of extract, respectively. TLC analysis using gallic acid and quercetin as marker compounds demonstrated characteristic chromatographic profiles. The aqueous extract exhibited spots with Rf values comparable to those of gallic acid and quercetin, indicating the probable presence of phenolic and flavonoid constituents. The results confirmed that Caesalpinia crista is a rich source of bioactive phytochemicals and provided valuable chromatographic fingerprints for its identification, authentication, and quality control. The study supports the medicinal significance of the plant and its potential for future pharmaceutical and nutraceutical applications.
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