PHYTOCHEMICAL PROFILING AND QUANTIFICATION OF SECONDARY METABOLITES IN SOYMIDA FEBRIFUGA EXTRACT USING UV AND HPLC TECHNIQUES
Aneesh Jaiswal, Virendra Kumar Sharma
ABSTRACT
The present study was aimed at phytochemical profiling and quantification of secondary metabolites present in the hydroalcoholic extract of Soymida febrifuga using UV spectrophotometric and HPLC techniques. The extract was evaluated for physical characteristics, percentage yield, qualitative phytochemical screening, Thin Layer Chromatography (TLC), total phenolic content, total flavonoid content, and quantitative estimation of quercetin by HPLC analysis. The hydroalcoholic extract showed a black solid consistency with a percentage yield of 7.60%. Preliminary phytochemical screening revealed the presence of flavonoids, diterpenes, phenols, proteins, carbohydrates, and saponins, while alkaloids, glycosides, tannins, and sterols were absent. TLC analysis confirmed the presence of quercetin with an Rf value of 0.58 corresponding to the standard compound. Quantitative estimation showed total phenolic content and total flavonoid content of 0.574 mg/100 mg and 0.806 mg/100 mg respectively. HPLC analysis demonstrated the presence of quercetin in the extract with a retention time of 2.553 min, closely matching the standard quercetin peak at 2.556 min. The assay indicated 0.15% quercetin content in the extract. The findings suggest that Soymida febrifuga is a rich source of biologically active secondary metabolites and the developed analytical methods are suitable for phytochemical standardization and quality evaluation of the plant extract.
[Full Text Article]