STUDY OF PHYTOCHEMICALS, IN VITRO ANTIMICROBIAL, ANTI INFLAMMATORY AND ANTI DIABETIC ACTIVITY OF COCHLOSPERMUM GOSSYPIUM EXTRACT
Km. Tripti Shrivastava, Gyan Singh, Vivek Gupta
ABSTRACT
The present study evaluated the phytochemical composition and in vitro antimicrobial, anti-inflammatory, and antidiabetic activities of the hydroalcoholic extract of Cochlospermum gossypium. The percentage yield of the extract was found to be 8.52%, indicating moderate extraction efficiency using a hydroalcoholic solvent system suitable for extracting both polar and semi-polar phytoconstituents. Phytochemical screening revealed the presence of alkaloids, flavonoids, phenols, proteins, carbohydrates, and diterpenes. The total phenolic and flavonoid contents were found to be 0.58 mg/100 mg and 0.96 mg/100 mg, respectively, suggesting a higher flavonoid concentration. The antimicrobial study demonstrated moderate activity against Bacillus subtilis and Klebsiella pneumoniae, with maximum zones of inhibition observed at higher concentrations. The anti-inflammatory activity showed a concentration-dependent increase, with 69.58% inhibition at 500 µg/ml, while the antidiabetic activity exhibited 62.58% inhibition at 150 µg/ml. Although the activities were lower than standard drugs, the extract showed significant pharmacological potential. These biological effects may be attributed to the presence of flavonoids and phenolic compounds. The study supports the traditional use of Cochlospermum gossypium and highlights its potential as a source of natural therapeutic agents. Further studies are required to isolate active constituents and confirm efficacy through in vivo investigations.
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