DEVELOPMENT OF EVALUATION OF ANTI-DIABETES POLYHERBAL TABLETS FORMULATION
Anupma Dhobi, Arpit Shrivastava, Prateek Kumar Jain, Harshita Jain
ABSTRACT
The present study was aimed at the development, formulation, and evaluation of a polyherbal tablet with antidiabetic potential containing ethanolic extracts of Combretum ovalifolium, Gardenia lucida, and Leea macrophylla. The selected plants were subjected to extraction, percentage yield determination, and preliminary phytochemical screening. Quantitative estimation of total phenolic and flavonoid contents was also carried out to identify bioactive constituents. The polyherbal tablets were formulated and evaluated for pre-compression and post-compression parameters, including bulk density, flow properties, hardness, friability, weight variation, drug content, and disintegration time. In vitro antidiabetic activity was assessed using enzyme inhibition studies and compared with the standard drug acarbose. The in vivo antidiabetic potential of the polyherbal tablets was evaluated in experimentally induced diabetic rats by monitoring body weight and blood glucose levels over a 21-day treatment period, with glibenclamide used as the standard drug. Preliminary phytochemical analysis revealed the presence of flavonoids, phenolic compounds, saponins, and proteins in the extracts. The formulated tablets exhibited acceptable physicochemical properties and rapid disintegration. In vitro studies demonstrated concentration-dependent enzyme inhibition by the polyherbal tablets, while in vivo studies showed significant improvement in body weight and a marked reduction in blood glucose levels, particularly at higher doses. The findings of the study suggest that the developed polyherbal tablet formulation possesses significant antidiabetic activity, which may be attributed to the synergistic effects of bioactive phytoconstituents. The formulation represents a promising herbal alternative for the management of diabetes mellitus.
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