EXTRACTION, PHYTOCHEMICAL INVESTIGATION AND QUANTITATIVE STUDY OF TPC AND TPC BY UV VIS. SPECTROSCOPY IN EXTRACT OF DIOSCOREA BULBIFERA
Anab Ahmad, Satkar Prasad, Sailesh Kumar Ghatuary
ABSTRACT
The present study focused on the extraction, phytochemical investigation, and quantitative analysis of total phenolic content (TPC) and total flavonoid content (TFC) of the hydroalcoholic extract of Dioscorea bulbifera leaves. The leaves were extracted using a hydroalcoholic solvent system by maceration, yielding 12.5% w/w of extract. Preliminary phytochemical screening revealed the presence of flavonoids, phenols, carbohydrates, saponins, and diterpenes. Thin-layer chromatography (TLC) further confirmed the presence of quercetin as one of the key flavonoid components. Quantification of TPC and TFC was carried out using UV-Visible spectrophotometry. The extract exhibited 0.461 mg GAE/100 mg of phenolic content and 0.875 mg QE/100 mg of flavonoid content, indicating strong antioxidant potential. The study provides a basis for the therapeutic application of Dioscorea bulbifera in oxidative stress and inflammatory disorders and supports its traditional medicinal use.
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