PHARMACOLOGICAL EVALUATION, ANTIOXIDANT AND ANTI-PYRETIC ACTIVITY OF ARCTIUM LAPPA ROOTS EXTRACT IN EXPERIMENTAL RATS
Nandni Prajapati, Harshita Jain, Arpit Shrivastava, Mansha Jain, Sunil Kumar Jain, Vaishali Yadav
ABSTRACT
The present study was conducted to investigate the phytochemical constituents, antioxidant, and antipyretic activities of Arctium lappa root extracts in experimental animal models. The plant roots were extracted sequentially using petroleum ether and methanol via Soxhlet extraction. Preliminary phytochemical screening revealed the presence of alkaloids and saponins in both extracts, while glycosides and carbohydrates were found only in the methanolic extract. Quantitative estimation showed that the methanolic extract contained significant levels of total phenolic content (61.66 mg GAE/g) and flavonoid content (17 mg RE/g). Antioxidant activity assessed by DPPH assay showed a dose-dependent radical scavenging effect, with an IC?? of 48.74 µg/mL, indicating moderate activity compared to ascorbic acid. The antipyretic effect was evaluated using Brewer’s yeast-induced pyrexia in Wistar rats. The methanolic extract at 200 mg/kg exhibited a significant reduction in rectal temperature, comparable to the standard drug paracetamol. The findings suggest that Arctium lappa root extract possesses promising antioxidant and antipyretic properties, potentially due to the presence of phenolic compounds and alkaloids.
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