PHYTOCHEMICAL ANALYSIS AND INDOMETHACIN INDUCED ANTIULCER ACTIVITY OF CATUNAREGAM SPINOSA
Neha Bharti, Manju Prajapati, Akhlesh Kumar Singhai
ABSTRACT
The present study was undertaken to evaluate the anti-ulcer potential of ethanolic extract of Catunaregam spinosa using an indomethacin-induced gastric ulcer model in rats, alongside comprehensive phytochemical screening, extract standardization, and assessment of bioactive contents. Phytochemical screening of Catunaregam spinosa revealed the presence of significant secondary metabolites, including flavonoids, phenols, alkaloids, tannins, and saponins, which are known for their gastroprotective and antioxidant properties. The extract demonstrated total phenolic and flavonoid content of 0.958 mg/100 mg and 0.647 mg/100 mg, respectively. In the in vivo anti-ulcer model, Catunaregam spinosa at 200 mg/kg significantly reduced the ulcer index (1.90 ± 0.55) and number of ulcers (2.35 ± 0.50) while elevating the gastric pH (5.75 ± 0.85), compared to the ulcer control group. The results were comparable to those obtained with the standard drug omeprazole (30 mg/kg). Catunaregam spinosa demonstrated significant anti-ulcer activity supported by both phytochemical richness and in vivo pharmacological evidence, indicating its potential as a natural therapeutic agent.
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