SYNTHESIS AND SCREENING OF NEWER BENZOTRIAZOLE DERIVATIVES FOR THEIR ANTIBACTERIAL AND ANTIOXIDANT POTENTIAL
Saksham Agrawal, Satyam Sing, Saurabh Dwivedi, Saurabh Kumar, Rakesh Gawaly
ABSTRACT
The present study focuses on the synthesis, characterization, and biological evaluation of novel benzotriazole derivatives for their antibacterial and antioxidant potential. A series of benzotriazole derivatives (BTZ-1 to BTZ-4) were synthesized via N-alkylation, Schiff base formation, and acylation reactions. The synthesized compounds were purified using recrystallization and column chromatography, and their purity was confirmed by Thin Layer Chromatography (TLC). Structural characterization was performed using FTIR, ¹H NMR, and ¹³C NMR spectroscopy, which confirmed the presence of key functional groups such as aromatic rings, imine linkages, and nitrogen-bearing side chains. The antibacterial activity was tested against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa using the agar well diffusion method. Among the synthesized compounds, BTZ-4 showed the most significant antibacterial activity, comparable to the standard drug ciprofloxacin. Antioxidant activity was assessed using the DPPH radical scavenging assay, where BTZ-4 again exhibited the highest scavenging activity with an IC?? of 39.6 µg/mL. These results suggest that benzotriazole derivatives, particularly BTZ-4, have promising potential as therapeutic agents with dual antibacterial and antioxidant activity.
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