PHYTOCHEMICAL INVESTIGATION AND THIN LAYER CHROMATOGRAPHY OF HYDROALCOHOLIC EXTRACT OF VACHELLIA NILOTICA
Abhishek Kumar Mishra, B. K. Dubey, Deepak Kumar Basedia
ABSTRACT
The present study aims to investigate the phytochemical composition and bioactive potential of Vachellia nilotica (formerly Acacia nilotica) through the analysis of its hydroalcoholic extract. The extract was obtained using a hydroalcoholic solvent system, yielding 7.50% of a dark black extract, which was subjected to preliminary phytochemical screening and Thin Layer Chromatography (TLC). Preliminary phytochemical screening revealed the presence of alkaloids, carbohydrates, flavonoids, and proteins, while glycosides, tannins, resins, and some other constituents were absent. TLC analysis of the flavonoid content demonstrated the presence of multiple flavonoid compounds with varying Rf values under different UV light conditions, suggesting a diverse flavonoid profile in the extract. These findings support the medicinal potential of Vachellia nilotica, particularly for its antioxidant, anti-inflammatory, and antimicrobial activities, which may be attributed to the alkaloids and flavonoids identified. The study contributes to the understanding of the plant's chemical composition, which may provide a foundation for further research into its therapeutic applications and the isolation of bioactive compounds for potential pharmaceutical development.
[Full Text Article]