PHYTOCHEMICAL SCREENING AND ANTI-OXIDANT ACTIVITY OF MEDICINAL PLANT CORDIA DICHOTOMA
Abraham Nartey, Omjay Manikpuri, Jaswinder Mehta, Ruchi Acharya, Bhawna Sharma, Peenu Mahendra Joshi
ABSTRACT
The medicinal plant Cordia dichotoma (Family: Boraginaceae) has been traditionally used for the treatment of various ailments, including digestive disorders, fever, respiratory infections, and inflammation. This study aims to evaluate the phytochemical composition and antioxidant activity of the hydroalcoholic extract of Cordia dichotoma leaves. Phytochemical screening revealed the presence of bioactive compounds, such as alkaloids, glycosides, flavonoids, phenols, proteins, tannins, and sterols. Thin layer chromatography (TLC) analysis confirmed the presence of flavonoids and phenolic compounds, with the Rf values corresponding to quercetin and gallic acid, respectively. The total phenol content of the extract was found to be 4.76 mg/100mg, and the total flavonoid content was 1.41 mg/100mg. The antioxidant activity was assessed using the DPPH radical scavenging assay, where the hydroalcoholic extract exhibited significant antioxidant activity, with 84.54% scavenging effect at a concentration of 100 ?g/ml. The IC50 value for the hydroalcoholic extract was 61.68 ?g/mL, indicating moderate antioxidant potential compared to the standard ascorbic acid (IC50 = 34.30 ?g/mL). These findings suggest that Cordia dichotoma leaves are a promising source of natural antioxidants and bioactive compounds with potential therapeutic applications in managing oxidative stress-related diseases.
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