CHARACTERIZATION OF FAGONIA ARABICA CONTACTING SILVER NANOPARTICLES GEL USING BOX BEHNKEN DESIGN
Arjun Singh, Govind Nayak, Richa Verma, Mehta Parulben D
ABSTRACT
The present study investigates the phytochemical, antimicrobial, and formulation optimization of Fagonia arabica, a plant known for its medicinal properties. The study focuses on the extraction of bioactive compounds from the aerial parts of Fagonia arabica using hydroalcoholic solvent, which yielded a higher percentage (9.20%) compared to pet ether extract (0.86%). Phytochemical screening revealed the presence of flavonoids, phenols, proteins, saponins, and tannins, all of which contribute to the plant’s therapeutic potential. In addition, the study explored the optimization of gel formulations containing silver nanoparticles to enhance bioactive compound delivery. The optimized formulation, F5, showed the highest yield (82.23%) and good encapsulation efficiency (0.780 mg/100 mg quercetin equivalent). The formulated gels exhibited favorable physical properties, such as smooth texture, good spreadability, and appropriate pH for topical use. The in vitro drug release study indicated a controlled release of the active compounds, with formulation F1 showing a sustained release up to 4 hours. Antimicrobial tests confirmed that the silver nanoparticle-based gels exhibited superior antimicrobial activity against Bacillus subtilis and Klebsiella pneumoniae, demonstrating the enhanced efficacy of the formulation. The results suggest that Fagonia arabica and its formulated gel preparations have significant potential for therapeutic applications, particularly in antimicrobial and dermatological treatments.
[Full Text Article]