IN-VIVO ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC LEAVES EXTRACT OF GARDENIA RESINIFERA
Archana Rai, Surendra Kumar Jain, K. K. Badoniya
ABSTRACT
This study evaluated the phytochemical composition and pharmacological activities of the ethanolic extract obtained from Gardenia resinifera leaves. The extraction process yielded 8.6% w/w of the ethanolic extract, indicating moderate efficiency. Phytochemical screening confirmed the presence of alkaloids (via Hager's and Wagner's tests), flavonoids, phenols, proteins, saponins, diterpenes, and tannins, indicating a diverse array of bioactive compounds. Quantitative analysis revealed the presence of 1.26 mg/100 mg of total flavonoids and 1.45 mg/100 mg of total phenols in the ethanolic extract. Antioxidant activity was assessed using the DPPH assay, with the extract exhibiting an IC50 value of 83.06 ?g/mL, demonstrating moderate antioxidant potential compared to ascorbic acid (IC50 = 34.57 ?g/mL). Additionally, in a carrageenan-induced paw edema model, the extract displayed dose-dependent anti-inflammatory effects, significantly reducing paw volume compared to the control group. This observed anti-inflammatory activity supports the traditional use of Gardenia resinifera in managing inflammatory conditions. These findings highlight Gardenia resinifera as a promising source of bioactive compounds with antioxidant and anti-inflammatory properties, prompting further research into its therapeutic applications.
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