International Journal of Pharmaceutics & Drug Research

Satendra, Deepak Tripathi, Ravi Kumar, Murari Lal, Avinash Krishnarao Kondalkar

ABSTRACT

This study investigates the development and characterization of a phytosomal formulation using the hydroalcoholic extract of Putranjiva roxburghii, a medicinal plant known for its bioactive properties. The extraction process yielded 2.65% from pet ether and 7.85% from a hydroalcoholic solvent, indicating that the hydroalcoholic extract is more effective in isolating the plant’s bioactive compounds. Phytochemical screening revealed the presence of alkaloids, glycosides, flavonoids, proteins, carbohydrates, saponins, and tannins, with significant antioxidant potential, primarily due to the flavonoid and phenolic content. Quantitative analysis of the hydroalcoholic extract showed a total phenolic content of 0.65 mg/100 mg and total flavonoid content of 0.85 mg/100 mg, underscoring the extract’s potential as a source of natural antioxidants for therapeutic applications. To enhance the bioavailability and stability of the active compounds, a phytosomal formulation was prepared by complexing the extract with a phospholipid complex in a 1:1:1 ratio of phospholipids, cholesterol, and Putranjiva roxburghii extract. Among the various formulations, F10 exhibited the smallest particle size (215.47 nm) and a high entrapment efficiency of 74.66%, indicating enhanced stability and bioavailability. The release kinetics followed a First Order model (R² = 0.9912), suggesting a concentration-dependent release, with diffusion also playing a key role, as confirmed by the Higuchi model (R² = 0.9544). Stability studies revealed that the optimized phytosome formulations remained stable for up to three months under controlled conditions, ensuring their potential for therapeutic use. The results emphasize the promising therapeutic applications of Putranjiva roxburghii in drug delivery systems and encourage further research into its bioactivity and clinical efficacy.

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