DEVELOPMENT AND VALIDATION OF SIMPLE COST EFFECTIVE UV AND HPLC METHOD FOR ESTIMATION PREGABALIN AND ETORICOXIB
Akshay Gulab Sonwane, Snehal R. Karmankar, Sachin B Dudhe
ABSTRACT
This study evaluates the performance of ultraviolet (UV) and high-performance liquid chromatography (HPLC) methods for the determination of Pregabalin (PGB) and Etoricoxib (EXB) in pharmaceutical formulations. Both methods were validated for linearity, accuracy, precision, and sensitivity. The UV method demonstrated excellent linearity with correlation coefficients (r²) of 0.999 for both PGB and EXB. The recovery rates were high, with PGB recovering between 98.21% and 98.57% and EXB between 95.08% and 96.62%. The precision of the UV method was confirmed through low %RSD values in various categories. In comparison, the HPLC method also exhibited high linearity (r² = 0.999) and accuracy, with recovery percentages ranging from 98.09% to 99.18% for PGB and 98.759% to 99.873% for EXB. The HPLC method showed higher sensitivity with lower LOD and LOQ values compared to UV, making it suitable for detecting trace amounts of the drugs. Both methods were effective in analyzing tablet formulations, with HPLC providing greater sensitivity and precision. This study highlights the strengths of each method and provides guidance on selecting the appropriate technique based on analytical requirements.
[Full Text Article]