METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF QUINAPRIL AND HYDROCHLOROTHIAZIDE USING UV-VIS SPECTROSCOPY
Dhanashri Bhumeshwar Yele, Snehal R. Karmankar, Sachin B. Dudhe
ABSTRACT
This study presents the development and validation of a UV-Vis spectroscopy method for the simultaneous estimation of Quinapril and Hydrochlorothiazide in pharmaceutical formulations. The method was optimized to identify the maximum absorbance wavelengths for Quinapril at 230.0 nm and Hydrochlorothiazide at 276.0 nm. Linearity was established over a concentration range of 5-25 µg/ml for both drugs, with high correlation coefficients (r² = 0.999). Accuracy was confirmed through recovery studies, yielding mean recovery percentages between 98.04% and 98.94%. Validation of the method showed excellent precision across repeatability, day-to-day, analyst-to-analyst, and reproducibility tests, with low relative standard deviations. The method demonstrated sensitivity with limits of detection of 0.15 µg/ml for Quinapril and 0.10 µg/ml for Hydrochlorothiazide, and limits of quantification of 0.45 µg/ml and 0.30 µg/ml, respectively. Analysis of tablet formulations confirmed the method’s applicability and accuracy, with mean recovery values of 99.53% for Hydrochlorothiazide and 98.29% for Quinapril. This validated UV-Vis spectroscopy method offers a reliable and efficient approach for the simultaneous quantification of Quinapril and Hydrochlorothiazide in quality control settings.
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