SPECTROSCOPIC AND HPTLC STUDY FOR QUALITATIVE AND QUANTITATIVE STUDY OF PHYTOCOMPOUND IN EXTRACT OF PUERARIA TUBEROSA
Agam Kumar, Virendra Kumar Sharma, Mrs. Sushma Somkuwar, Akhlesh Kumar Singhai
ABSTRACT
The present study investigates the qualitative and quantitative phytochemical composition of the ethanolic extract of Pueraria tuberosa. The extraction yielded 7.2% (w/w) of the dried extract. Comprehensive phytochemical screening revealed the presence of flavonoids, phenols, proteins, saponins, and diterpenes, while alkaloids, glycosides, carbohydrates, and tannins were absent. Quantitative analysis indicated significant amounts of total phenols (0.622 mg/100 mg) and flavonoids (0.835 mg/100 mg) in the extract. Thin Layer Chromatography (TLC) and High-Performance Thin Layer Chromatography (HPTLC) confirmed the presence of quercetin, a potent antioxidant, with a concentration of 0.75%. These findings underscore the potential therapeutic value of Pueraria tuberosa, warranting further investigation into its pharmacological applications.
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