ISOLATION & STRUCTURAL CHARACTERIZATION OF BIOACTIVE COMPOUND IN HERBAL MEDICINAL PLANT PTEROCARPUS MARSUPIUM
Sudhanshu Kumar, Virendra Kumar Sharma, Mrs. Sushma Somkuwar, Akhlesh Kumar Singhai
ABSTRACT
The study aimed to isolate and structurally characterize bioactive compounds from the herbal medicinal plant Pterocarpus marsupium. The extraction process utilized solvents of varying polarities, resulting in a petroleum ether extract (yielding 1.2% w/w) and a hydroalcoholic extract (yielding 8.5% w/w). Phytochemical screening of the hydroalcoholic extract indicated the presence of alkaloids, flavonoids, phenols, carbohydrates, saponins, and diterpenes, while glycosides, proteins, and tannins were absent. Quantitative analysis revealed total flavonoid and phenol contents of 0.852 mg and 0.523 mg per 100 mg of dried extract, respectively. Structural characterization through IR spectroscopy, 1H NMR spectroscopy, and mass spectrometry identified the isolated compound as 3,5,7-trihydroxy-2-(4-hydroxyphenyl) chromen-4-one, a flavonoid. This study highlights the significant presence of bioactive flavonoids in Pterocarpus marsupium, suggesting its potential therapeutic applications. Further investigations are warranted to explore the bioactivity and pharmacological properties of the identified compound.
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