UV AND HPLC ESTIMATION OF SECONDARY METABOLITE PRESENT IN EXTRACT OF PLUMBAGO ZEYLANICA ROOT
Ashutosh Kushwaha, Virendra Kumar Sharma, Sushma Somkuwar,
Akhlesh Kumar Singhai
ABSTRACT
Plumbago zeylanica (Ceylon leadwort) is a medicinal plant traditionally used for its therapeutic properties. The roots of Plumbago zeylanica are known to contain various bioactive compounds with pharmacological activities. This study aimed to evaluate the hydroalcoholic extract of Plumbago zeylanica root for its phytochemical composition and quantify the presence of quercetin using high-performance liquid chromatography (HPLC). The hydroalcoholic extract of Plumbago zeylanica root was prepared and subjected to phytochemical screening for alkaloids, glycosides, flavonoids, phenols, proteins, carbohydrates, saponins, diterpenes, and tannins. Total phenol and flavonoid content was quantified spectrophotometrically. HPLC was employed to quantify quercetin in the extract. The hydroalcoholic extract showed the presence of flavonoids, phenols, proteins, saponins, diterpenes, and tannins, while alkaloids and glycosides were absent. The extract contained 0.674 mg of total phenols and 0.793 mg of total flavonoids per 100 mg of extract. Quercetin was identified and quantified with a retention time of 2.569 minutes and an assay percentage of 0.075%.The study confirms that the hydroalcoholic extract of Plumbago zeylanica root is rich in bioactive compounds, particularly flavonoids and phenols. Quercetin, a known antioxidant and anti-inflammatory compound, was identified in the extract. These findings support the traditional use of Plumbago zeylanica in herbal medicine and suggest its potential for further therapeutic applications.
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