PHYTO-CONSTITUENT AND SCREENING OF ANTIHYPERGLYCEMIC ACTIVITY OF ALANGIUM LAMARCKII ROOTS EXTRACT
Ashutosh Mishra, Rameshwar Ahirwar, Anita Shivhare Vijay Nigam, Pawan Bajpai, Sanjay Jain
ABSTRACT
Diabetes is a serious complex condition which can affect the entire body. Diabetes requires daily self care and if complications develop, diabetes can have a significant impact on quality of life and can reduce life expectancy. While there is currently no cure for diabetes, you can live an enjoyable life by learning about the condition and effectively managing it. It is estimated that in 2010 there were globally 285 million people (approximately 6.4% of the adult population) suffering from this disease. The present study is undertaken to standardize the Alangium lamarckii and to evaluate its anti-diabetic activity with emphasize to its mechanism of action. The validation of anti-diabetic property is correlated with various biochemical parameters involved therein. The in-vivo antidiabetic activity of ethanolic extract of Alangium lamarckii root was tested by using an Alloxan induced diabetic rat. Acute toxicity study was carried out in rats. The procedure was followed by OECD 423 (Acute toxicity class method). 1/5th (400mg/kg) of the maximum safe dose (2000mg/kg) were selected for further study. Fasting blood sample were drawn from tail vein of rats at weekly intervals till the end of the study 1,7 and 14 days. On these days fasting blood glucose were collected and analysed for glucose. At the end of the study (14th day) the ethanolic extract of Alangium lamarckii root (400 mg/kg p.o) treated diabetic groups showed statistically significant decrease in blood glucose similar to the standard drug glibenclamide (2mg/kg), which indicated block the alfa amyalase activity. The present study suggested that the isolation of active constituents from ethanolic extract of Alangium lamarckii root and characterize the compounds by using preliminary phytochemical studies.
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