EXTRACTION, PHYTOCHEMICAL ANALYSIS AND ANTI DIABETIC ACTIVITY OF ARABOTRYS HEXAPETALUS ROOTS
Rakshika Prajapati, Dr. Vishnu Raj, Dr. Brijesh Sirohi, Dr. Shailendra Lariya
ABSTRACT
Diabetes continues to be a major contributor to cardiovascular disease, lower limb amputation, end-stage renal disease, and blindness. Due to the negative effects of oral hypoglycemic drugs (therapeutic agents) for the treatment of diabetes mellitus, there is currently an increasing interest in herbal therapies. Thus, this study deals with extraction, phytochemical analysis and anti-diabetic activity of Arabotrys hexapetalus roots. The roots of plant gathered and subjected to further processing. After qualitative antd quantitative studies the extract anti diabetic effect was checked in STZ induced diabetic rats. Results showed that the % yield of pet. ether and ethanolic extract as 2.3% and 5.8% respectively. The phytochemical screening revealed the presence of carbohydrates, amino acid, and proteins, Flavonoids, Tannins and Phenol, Alkaloids. The total phenol and alkaloid content in ethanolic extract was observed to be 0.756 & 0.632mg/100mg respectively. Further, the body weight and blood glucose level was continuously monitored to check the antidiabetic effect in animal models. It was observed that, in diabetic control the body weight was reduced to 125.2±6.38gm on 21st day. While in Glibenclamide 0.25 mg/kg treated rats the final weight was noted to be 126.2±4.76gm. Further in EEAH (50 mg/kg), EEAH (100 mg/kg) and EEAH (100 mg/kg) treated rats the body weight was measured to be 122.7±3.89gm, 134.7±7.84gm and 137.4±4.88gm respectively. Further, the changes in blood glucose level in all groups was carefully observed. The blood glucose in Glibenclamide (0.25 mg/kg) treated rats was estimated to be 153.8±4.44 mg/dl at 21st day. In case of EEAH (50 mg/kg), EEAH (100 mg/kg) and EEAH (200 mg/kg) treated rats the blood glucose level detected to be 191.3±4.36, 189.2±7.48 mg/dl, 168.66±4.47 mg/dl respectively. On the basis of results of this preliminary study it is concluded that ethanolic extract of Arabotrys hexapetalus roots has dose-dependent anti-diabetic activity. The present study shows that the Arabotrys hexapetalus roots extract at dose 200 mg/kg has significant anti-diabetic activity.
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