CHROMATOGRAPHY AND SPECTROSCOPY TECHNIQUES USED IN THE AUTHENTICATION AND ANALYSIS OF CANNABIS SATIVA A MEDICINAL PLANT
Rajashree Chavan, Ganesh Nigade, Suneja Mane, Prajakta Mane, Neha Gambhire, Aditi Patil
ABSTRACT
Ayurveda uses a variety of bioactive compounds, which are abundant in plants, to cure a variety of diseases. Since ancient times, people have used medicinal herbs, and it's possible to say that this practice is where modern medicine got its start. There is an urgent need for herbal remedies to be assured of both their safety and effectiveness as medicinal plants keep increasing in popularity across the world. With this increasing need, it is very essential that the quality of the herbal medicinal plant must be controlled. In particular, the analysis of medicinal herbs has been around for decades to determine a plant's quality. There are many spectroscopic and chromatographic methods available for analysis, including ultraviolet (UV), Fourier-transform infrared spectrum, NMR spectroscopy or magnetic resonance spectroscopy (MRS), vapor-phase chromatography (VPC), high-pressure liquid chromatography (HPLC) along with mass spectrometry (MS), as well as hyphenated methods like vapor-phase chromatography (VPC)-mass spectrometry, liquid chromatography-hyphenated methods, and liquid chromatography-hyphenated methods. Cannabis sativa is a medical plant that is attracting more attention as a result of its strong pharmacological potential and recent changes to the law that permit diverse applications. For phytocannabinoid profiling, it is essential to create analytical techniques that are both time and money effective. The study intends to demonstrate the applicability of methodology for phytocannabinoid profiling of cannabis in addition to explore new analytical approaches in cannabis quality control, including classical spectroscopic as well as chromatographic methods.
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