PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF HYDROALCOHOLIC EXTRACT OF ANACYCLUS PYRETHRUM
Wasiullah, Mr. Bhushan Korde, Dr. B. K. Dubey
ABSTRACT
In this investigation, the antioxidant capacity of Anacyclus pyrethrum was assessed. The plant material was collected, extracted & subjected for qualitative & quantitative study. Through the use of DPPH free radical, nitric oxide radical inhibition, Hydrogen peroxide method, antioxidant activity was additionally, the amounts of total phenolics, flavonoids, and proanthocyanidin were measured. Results showed that the percentage loss of Anacyclus pyrethrum was found to be 17.8%. The % yield was observed to be 1.65% and 5.21% in pet ether & hydroalcoholic extract respectively. The flavonoids, saponins, proteins, carbohydrate, phenol, alkaloids and diterpenes were present in hydroalcoholic extract of Anacyclus pyrethrum. The total phenol & flavonoid content was observed to be 0.397 and 0.733 mg/100mg respectively. The IC 50 value for hydroalcoholic extract was seen to be 67.78 for DPPH assay, the standard ascorbic acid IC50 value was estimated to be 18.69. While the IC50 value obtained by NO method is 87.33% for the extract & IC50 value of ascorbic acid in this case is 18.69%. Further, by hydrogen peroxide method the IC50 value obtained for extract was 88.03% with comparison to IC50 value of 36.75 for ascorbic acid. Thus, Studies have shown that Anacyclus pyrethrum extracts have the ability to scavenge free radicals, reduce lipid peroxidation, and scavenge superoxide radicals. This suggests that Anacyclus pyrethrum may have antioxidant activity, which could be beneficial in treating oxidative stress-related diseases.
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