INVESTIGATING OF NANOCARRIERS INVASOMES OF METRONIDAZOLE FOR SKIN DRUG DELIVERY FOR TREATMENT OF MICROBIAL DISEASE
Karan, Anshita Gupta, Dr. Vivek Gupta
ABSTRACT
Metronidazole is a commonly used antibiotic. It is available in capsule form, tablet form, and topical form, and suppository preparations for the treatment of various infections. To increase its efficacy this study aims at formulation of invasomal gel of metronidazole. In total six formulations of invasomes were created the average vesicle size varied from 236.65±0.32 to 265.58±0.14 nm. The entrapment efficiency was observed to be varied from 66.45±0.32% to 98.85±0.32%. It was seen that for F5 formulation the average vesicle size and % entrapment efficiency as 210.32±0.14nm &74.65±0.26% respectively. Further three formulations of gel were created the viscosity of gel varied from 3165.37 to 3452.27. The pH of formulation ranged from 6.65 to 6.85. The spreadibility of formulation lies in between 11.32 to 13.32 g.cm/sec. The drug content was found to be maximum for IG-2 formulation which is 99.25% respectively. The extrudability varied from 169.98 to 180.32. In 12 hours, about 99.15% drug is released in case of IG-1. Further the study of In-vitro drug release data for optimized formulation IG-2 was seen to be 98.78%. The formulation IG-2 follows, 0.985 Higuchi model. With R2 value 0.985 In case of optimized gel formulation IG-2 at 100 ?g/ml the zone of inhibition against Escherichia coli and Staphylococcus aureus was found to be 16±0.47 mm and 14±0.94mm respectively. Here, in this case it can be deduced that invasomal gel also shows more potent antimicrobial activity against E. coli than S. aureus. The stability studies of invasomal gel revealed that formulation IG -2 can remain stable for three months when kept at 4.0 ±0.2°C with normal physical appearance and 99.05% drug content. Thus, the formulated invasomal gel of metronidazole can be effectively used for topical drug delivery.
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