UV METHOD DEVELOPMENT FOR THE ESTIMATION OF ANGIOTENSIN RECEPTOR-NEPRILYSIN INHIBITOR USING HYDROTROPY
Uma Bharti, Mr. Deepak Kumar Jain, Mr. Shankar Singh, Dr. Avinash K Kondalkar, Sapna Avinash Kondalkar, Mr. Muraree Lal
ABSTRACT
This research focuses on the development of a UV spectrophotometric method for the simultaneous estimation of Sacubitril and Valsartan in a pharmaceutical formulation. The proposed method leverages the concept of hydrotropy to enhance the solubility of the analytes, allowing for efficient and cost-effective analysis. The UV method was optimized using a double-beam UV spectrophotometer, and various hydrotropic agents were explored to enhance the solubility of Sacubitril and Valsartan in the UV range. The selection of a suitable hydrotropic agent was based on its ability to form a complex with the drugs, thereby increasing their solubility and improving the sensitivity of the UV measurement. The absorption spectra of Sacubitril and Valsartan were recorded, and the method's specificity was assessed by analyzing a combination of the two drugs. The calibration curves were constructed, and the method's linearity, precision, accuracy, and robustness were thoroughly evaluated. The developed UV method demonstrated excellent linearity over a specified concentration range, with good precision and accuracy. Additionally, the proposed method was successfully applied to the analysis of commercially available pharmaceutical formulations containing Sacubitril and Valsartan. The results obtained were statistically compared with those of a reference method, confirming the reliability and applicability of the developed UV method for routine quality control analysis. In conclusion, the developed UV spectrophotometric method utilizing hydrotropy provides a simple, cost-effective, and eco-friendly approach for the simultaneous estimation of Sacubitril and Valsartan. The method offers a viable alternative for routine analysis in pharmaceutical laboratories, contributing to the efficient quality control of formulations containing these two important cardiovascular drugs.
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