PHYTOCHEMICAL ANALYSIS, IN VITRO PHARMACOLOGICAL EVALUATION OF HYDROALCOHOLIC EXTRACT OF ARTOCARPUS HETEROPHYLLUS AND MURRAYA KOENIGII
Lokesh Sen*, Dr. C. K. Tyagi
ABSTRACT
The objective of this study is to decipher pharmacological potential of Fruits of Artocarpus heterophyllus and leaves of Murraya koenigii. The plant material was collected subjected to extraction. Further the qualitative, quantitative & in vitro pharmacological activities of both the plant was analyzed. Results showed that phytochemical screening revealed the presence of alkaloid, flavonoid, phenol, protein, and carbohydrate, saponins while the Murraya koenigii extract found to have flavonoid, phenol, carbohydrate, saponin & tannin. The total phenol & flavonoid content in Artocarpus heterophyllus was found to be 0.874 and 0.921 mg/100mg respectively. In case of Murraya koenigii the total phenol & flavonoid content was found to be 0.963 & 1.052 mg/100mg respectively. The antioxidant activity was performed by DPPH method the IC 50 value was observed to be 111.58 and 89.60 for Artocarpus heterophyllus and Murraya koenigii. The zone of inhibition against S. aureus & Bacilus subtilis at 100mg/ml concentration was found to be 10±0.47 and 11±1.24mm for Artocarpus heterophyllus extract. Also, the zone of inhibition against S. aureus & Bacilus subtilis at 100mg/ml concentration was found to be 12±0.47 and 11±0.57 mm for Murraya koenigii extract. Further antidiabetic assay suggested that the IC50 value of the Murraya koenigii extract (436.30 µg/ml) was lower than that of the Artocarpus heterophyllus extract (598.64 µg/ml), suggesting that the curry leaves extract has stronger alpha amylase inhibitory activity. The result of anti-inflammatory activity suggested that the IC50 values of the plant extracts were higher Artocarpus heterophyllus 248.87 µg/ml, Murraya koenigii 213.84 µg/ml), suggesting that they may have less potent antiinflammatory effects compared to the standard drug.
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