EXTRACTION, PHYTOCHEMICAL ANALYSIS AND ANTIDIABETIC ACTIVITY OF LITSEA GLUTINOSA
Arvind Kumar*, Dr. Satkar Prasad
ABSTRACT
Diabetes is anticipated to affect 438 million people (7.8% of the adult population) by 2030, according to recent estimates. The current medications for diabetes have a number of undesirable side effects. The Litsea glutinosa plant has numerous therapeutic characteristics and is traditionally used for a variety of alignments. The aim of this study is analyzing anti diabetic effect of Litsea glutinosa. The plant material was collected & subjected to hydroalcohlolic extraction followed by qualitative & quantitative analysis. The In vivo antidiabetic study was then carried out in STZ induced diabetic rats. The hydroalcoholic bark extract of Litsea glutinosa was given to particular group of rats in 100mg/kg & 200mg/kg of concentration. Glibenclamide was used as standard. Results revealed that Phytochemical screening revealed presence of Alkaloids, Carbohydrates, Glycosides, Saponins, Phenols, Flavonoids, Proteins. Total alkaloid & phenol content was found to be 0.954 mg/100mg 0.417 mg/100mg. The blood glucose level in in 100 mg/kg & 200mg/kg on 21st day was found to be 214.1±1.52 & 170.8±1.66 mg/dl. While in case of Glibenclamide it was observed to be 150.3±1.85mg/dl. The total cholesterol level in 100 mg/kg & 200mg/kg extract treated rats was observed to be 124.0 ± 1.50 & 109.1 ± 1.30 mg/dL respectively. For Glibenclamide, treated rats total cholesterol content in blood was noted to be 101.4 ± 1.10. Further the level of triglyceride in 100 mg/kg & 200mg/kg extract treated rats was seen to be 124.00 ± 1.80 &114.00 ± 1.80 mg/dL respectively. The protein content in 200 mg/kg extract group was observed to be 7.43 ± 0.60 g/dL. The final weight of rats in hydroalcoholic bark extract of Litsea glutinosa (200 mg/kg) was observed to be 185.00 ± 1.40. In summary, the blood glucose levels seen after treating diabetes-induced rats with hydroalcoholic extract of Listea glutinosa were equivalent to those obtained after treatment with glibenclamide.
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