International Journal of Pharmaceutics & Drug Research

Ravishankar Patel*, Julekha Siddiqui, Dr. Kavita R. Loksh

ABSTRACT

Aprepitant is a widely used drug for the prevention of chemotherapy-induced nausea and vomiting. The present study focuses on the development and validation of a simple and ecofriendly method for the estimation of aprepitant using mixed hydrotropic phenomena. The proposed method offers an alternative to traditional analytical techniques, reducing the use of hazardous organic solvents and promoting green analytical chemistry principles. The method development involved the optimization of various parameters, including the concentration of hydrotropic agents, pH of the solution, and temperature. Aprepitant was found to exhibit good solubility in a mixed hydrotropic solution composed of sodium benzoate and sodium acetate. The UV-visible spectrophotometric analysis was performed at the wavelength of maximum absorption (?max) determined using a standard aprepitant solution. The proposed method was validated as per International Conference on Harmonization (ICH) guidelines. The validation parameters such as linearity, accuracy, precision, specificity, robustness, and limits of detection and quantitation were evaluated. The method exhibited excellent linearity over the concentration range of aprepitant, with a correlation coefficient (r2) of >0.99. The accuracy of the method was confirmed by recovery studies conducted at different concentration levels, with percentage recoveries ranging from 98 to 102%. The precision of the method, assessed in terms of repeatability and intermediate precision, yielded relative standard deviation (RSD) values below 2%. The developed method offers several advantages, including simplicity, cost-effectiveness, reduced analysis time, and the use of ecofriendly hydrotropic agents. It provides a reliable and accurate estimation of aprepitant, making it suitable for routine analysis in pharmaceutical laboratories. The proposed method aligns with the principles of green chemistry, contributing to sustainable and environmentally friendly analytical practices.

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