IDENTIFICATION OF BIOACTIVE CONSTITUENTS OF ASPARAGUS RACEMOSUS ROOT EXTRACT USING UV AND HPLC
Vinod Lodhi1, Dr. Vivekanand Katare*1, Mrs. Abhilasha Delouri1, Mr. Prabhat Kumar Jain 2
ABSTRACT
Herbal medicines and their preparations have been widely used traditionally, for the thousands of years in developing and developed countries owing to its natural origin and lesser side effects or dissatisfaction with the results of synthetic drugs. One of the characteristics of oriental herbal medicine preparations is that all the herbal medicines, either presenting as single herbs or as collections of herbs in composite formulae A. racemosus is frequently used in ayurvedic drug preparations as it is known to treat conditions such as ageing, to boost immunity, improve longevity, vigor, mental function. A. racemosus also finds its application in curing neurological disorders, hepatopathy, tumors and dyspepsia. This study identified bioactive constituents of Asparagus racemosus. Results showed that the % Yield of roots extract of Asparagus racemosus was 42.76%. The Rf value of hydroalcoholic extract of Asparagus racemosus was found 0.31, 0.6, 0.7 indicates the presence of alkaloids. Data shows that amount of total flavonoids and alkaloid in hydroalcoholic extract was found 0.543 and 0.897 mg/100mg of dried extract. Quantitative estimation of alkaloid in hydroalcoholic extract of Asparagus racemosus was found 0.984% using HPLC.
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