PHYTOCHEMICAL INVESTIGATION AND IN VITRO ANTIDIABETIC ACTIVITY OF HYDROALCOHOLIC EXTRACT OF HIBISCUS LEAVES
Roli Shukla*
ABSTRACT
Nature has been a source of medicinal agents for thousands of years and a striking number of modern drugs have been isolated from natural source, many based on their use in traditional medicines or phytomedicines. Present investigation aim to access the in vitro antidiabetic activity of Hibiscus leaves extract against human pathogens. The content of total phenolic compounds (TPC) and total tannin content was expressed as mg/100mg of gallic acid equivalent of dry extract sample using the equation obtained from the calibration curve: Y = 0.011X+0.011, R2= 0.998, where X is the absorbance and Y is the tannic acid equivalent (GAE). Total flavonoids content was calculated as quercetin equivalent (mg/g) using the equation based on the calibration curve: Y=0.040X + 0.009, R2=0.999, where X is the absorbance and Y is the quercetin equivalent (QE). The total phenolic and flavonoids content of the hydroalcoholic extracts were also determined. The results of the present study intigate that the Hydroalcoholic extract of Hibiscus of ?-amylase showed maximum antidiabetic activity. Hence the extract may be useful as better therapeutic agent especially for the treatment of diabetes mellitus.
[Full Text Article]