QUALITATIVE AND QUANTITATIVE DETERMINATION OF SECONDARY METABOLITES AND ANTIOXIDANT POTENTIAL OF LEPTADENIA RETICULATA (RETZ.) WIGHT & ARN LEAF EXTRACTS
Roli Shukla*
ABSTRACT
Plants and plant-based medicaments are the basis of many of the modern pharmaceuticals we use today for our various diseases. Jivanti (Leptadenia reticulata (Retz.) is a well-known climber used for its innumerable therapeutic properties like antioxidant, antibacterial, vasodilator, galactagogue, Jivaniya etc., Its use in veterinary practice is tremendous due to its lactogenic effect. The aim of the present study was to determine qualitative and quantitative phytochemical and in vitro antioxidant activities of leaf of L. reticulata collected from Bhopal region of Madhya Pradesh. Qualitative analysis of various phytochemical constituents and quantitative analysis of total flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of flavonoids was carried out by aluminium chloride method. The in vitro antioxidant activity of hydroalcoholic extract of the leaf was assessed against DPPH assay method using standard protocols.Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids. The total flavonoids content of leaves hydroalcoholic extract was 1.943mg/100mg. The activities of hydroalcoholic leaves extract against DPPH assay method were concentration dependent with IC 50 values of ascorbic acid and extracts 14.11and 68.88?g/ml respectively. These studies provided information for standardization and correct identification of this plant material. The diverse array of phytochemicals present in the plant thus suggests its therapeutic potentials which may be explored in drug manufacturing industry as well as in traditional medicine.
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