EVALUATION OF INVIVO ANTI-INFLAMMATORY ACTIVITY OF HYDROALCOHOLIC LEAVES EXTRACT OF SACCOPETALUM TOMENTOSUM
Arvind Kumar Verma*, C. K. Tyagi, Prabhakar Budholiya
ABSTRACT
The present work showed phytochemical screening, anti-inflammatory activities and sub-acute toxicity of hydro-ethanolic extract of leaves of Saccopetalum tomentosum. The total flavonoid content (TFC) was expressed as mg/100mg of quercetin equivalent of dry extract sample using the equation obtained from the calibration curve: Y=0.032X + 0.018, R2=0.998, where X is the quercetin equivalent (QE) and Y is the absorbance. The total alkaloid content (TAC) was expressed as mg/100mg of Atropine equivalent of dry extract sample using the equation obtained from the calibration curve: Y=0.007X+ 0.024, R2=0.995, where X is the atropine equivalent (AE) and Y is the absorbance. Total alkaloid and flavonoids (mg/100mg) was found in hydroalcoholic extract of Saccopetalum tomentosum 1.635 and 0.957 mg/100mg respectively. Evaluation of the anti-inflammatory activity of the Hydroalcoholic Extract of leaves of Saccopetalum tomentosum was performed using the formalin-induced rat paw oedema model using diclofenac sodium as the reference drug. Mean changes in paw oedema thickness of animals treated with the tested compounds from induction of inflammation was measured, together with the inhibition percent of oedema by the tested extracts at 2 dose level 100mg/kg and 200mg/kg. Results showed that the tested extract 200mg/kg was found more active.
[Full Text Article]