STUDY OF PHYTOCHEMICAL INVESTIGATION AND IN VITRO ANTIOXIDANT POTENTIAL OF HYDROALCOHOLIC EXTRACT OF BARLERIA PRIONITIS
Dr. Roli Shukla
ABSTRACT
The objectives of this study are to screen the phytochemicals, estimate the content of phenolic and flavonoid compounds and determine the antioxidant capacity of the Barleria prionitis flower. Barleria prionitis is a famous perennial plant commonly known as porcupine flower or Vajradanti. It is a shrub with yellow flowers and two flat seeds shielded with matted hairs, inhabit most parts of India. Various parts of the plant such as leaves, roots, aerial parts, flowers, and stems are used in the traditional system of medicine. Conventionally, various infusions are prepared using the plant parts and utilized for the treatment of different kinds of diseases. The hydro alcoholic extract of flower of Barleria prionitis was studied for antioxidant activity on different in vitro models namely 1,1-diphenyl, 2-picryl hydrazyl (DPPH) assay. Phytochemical analysis revealed the presence of Alkaloid, Glycosides, phenols and flavonoids.. Ascorbic acid used as standards was also evaluated for comparison. The extract showed dose dependent free radical scavenging property in the tested models. Barleria prionitis flower extract showed IC50 value 51.32?g/ml for DPPH method, which was comparable to that of ascorbic acid (IC50=18.01?g/ml). The present study describes the phytochemical profile and antioxidant activity of Barleria prionitis which will further used for medicinal applications.
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