Preliminary Phytochemical Screening and In Vitro Antioxidant Activity of Hydroalcoholic Extract of Tinospora Cordifolia
Sri Prakash Mishra1*, Alok Pal Jain2
ABSTRACT
The aim of the present study was to determine qualitative and quantitative phytochemical and in vitro antioxidant activities of aerial part of T. cordifolia collected from Bhopal region of Madhya Pradesh. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by folins ciocalteau reagent method and aluminium chloride method respectively. The in vitro antioxidant activity of hydroalcoholic extract of the aerial part was assessed against DPPH assay method using standard protocols. Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenol, proteins, carbohydrates, saponins and diterpins. The total phenolic and flavonoids content of T. cordifolia aerial part of hydroalcoholic extract was 0.947 and 1.256 mg/100mg respectively. The activities of hydroalcoholic aerial part extract against DPPH assay method were concentration dependent with IC50 values of ascorbic acid and extract 17.68 and 79.20?g/ml respectively. These studies provided information for correct identification of this plant material. The diverse array of phytochemicals present in the plant thus suggests its therapeutic potentials which may be explored in drug manufacturing industry as well as in traditional medicine.
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