IJPDR

International Journal of Pharmaceutics & Drug Research

ISSN No. 2347-6346

Abstract

Validated Spectrophotometric and HPLC Method for the Estimation of Stavudine in Synthetic Mixture

Vivek Jain1, Jyoti Kirar*1, Geeta Parkhe2

ABSTRACT

Stavudine is an antiretroviral medication used to prevent and treat HIV/AIDS. It is generally recommended for use with other antiretroviral. It may be used for prevention after a needle stick injury or other potential exposure. However, it is not a first-line treatment. In present study a simple, sensitive and accurate isocratic reverse phase high performance liquid chromatography (RP-HPLC) and UV spectrophotometric method was developed for determination of stavudine in synthetic mixture. Different analytical performance parameters such as linearity, precision, accuracy, specificity, limit of detection (LOD) and limit of quantification (LOQ) were determined according to International Conference on Harmonization ICH Q2B guidelines. The RP-HPLC method was developed by the isocratic technique on a reversed-phase Thermo C18 (250 × 4.6 mm, 5µm) column with mobile phase consisting of methanol: acetonitrile (50:50v/v) at flow rate of 1.0ml/min. The retention time for stavudine was 2.715+0.3min. The UV spectrophotometric determinations were performed at 266 nm using water as a solvent. The linearity range for stavudine was 525?g/ml for both HPLC and UV method. The linearity of the calibration curves for each analyte in the desired concentration range was good (r2 >0.999) by both the HPLC and UV methods. The method showed good reproducibility and recovery with percent relative standard deviation less than 2%. Moreover, the accuracy and precision obtained with HPLC co-related well with the UV method which implied that UV spectroscopy can be a cheap, reliable and less time consuming alternative for chromatographic analysis.

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