ANTI-ARTHRITIC ACTIVITY OF HYDRO-ALCOHOLIC EXTRACT OF LEAVES OF ZIZIPHUS MAURITIANA
Sudheer Vishwakarma1, Narendra Patel1, Hemant Kumar Sharma1, Prabhakar Budholiya1, Prabhat Jain2
ABSTRACT
To investigate anti-arthritic activity of hydroalcoholic extract of Ziziphus mauritiana (HAZM) in Freund’s complete adjuvant (FCA)-induced arthritis in rats. The leaves the plant was collected, dried and extracted (maceration) with 70% methanol. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. The HAZM was prepared and subjected to acute oral toxicity in mice and tested against FCA induced arthritis in rats. Arthritis assessment was done by measuring – paw volume with standard reference drug diclofenac sodium was administered at the dose of 200 and 400 mg/kg body weight. HAZM dose dependently showed anti-arthritic activity which was evident with decrease in paw volume when compared to arthritic control group. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids, fixed oil and fats. The total phenolics content of leaves hydroalcoholic extract was (0.658 mg/100mg), followed by flavonoids (0.514mg/100mg). The results reveal promising anti arthritic potential of the plant. However further pharmacological investigation using isolated active ingredients can be carried out to confirm its efficacy and mechanism of action.
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