ESTIMATION OF BIOACTIVE CONSTITUENT OF HYDROALCOHOLIC EXTRACT OF ZIZIPHUS MAURITIANA USING RP-HPLC
Dr. Shailja kamle1, Geeta Parkhe*2, Sangeeta Balbase1, Shraddha Nagarare1
ABSTRACT
Ziziphus mauritiana is one of the underutilized herbs having potential to heal various ailments. It is reported in the ancient literature that whole plant as fruits, leaves, seed and root possess pharmacological activity. Several polyphenols have been tested in Ziziphus mauritiana. The flavonoid content is important because of the pharmacological properties of these compounds, whereas quercetin has been proved to be an antioxidant, antiinflammatory and hepatoprotective compound. A simple reverse phase highperformance liquid chromatography (RP-HPLC) method for the separation and quantitative determination of bio active constituent of hydroalcoholic extract of Ziziphus mauritiana has been developed and validated. The use of aC-18 column with mobile phase enabled the efficient separation of bio active constituent of hydroalcoholic extract within a 30 min analysis. The RP-HPLC method was validated as per ICH guidelines. The proposed RP-HPLC method was found to be simple, precise and accurate.
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