PHYTOCHEMICAL ANALYSIS, ANTIOXIDANT, ANTIDIABETIC AND IN VIVO ANALGESIC ACTIVITY OF CAESALPINIA BONDUCELLA
Uzma Ishrat Khan*1, Shailendra Kumar Lariya1, Vishwakarma Singh1, Prabhat Jain2
ABSTRACT
Caesalpinia bonducella Fleming (Caesalpiniaceae) plant is well known for its medicinal and therapeutic values in Indian Ayurveda. However, to be clinically useful, more scientific data are needed. Therefore, in the present study, we investigated the antioxidant, antidiabetic and in vivo analgesic activities of hydroalcoholic extract of C. bonducella leaves. Qualitative analysis of various phytochemical constituents, quantitative analysis of total phenolics (Folins ciocalteau reagent method) and flavonoids (Aluminium chloride method), in vitro free radical scavenging activity (DPPH radical method), in vitro anti-diabetic activity (Inhibition of alpha amylase enzyme) and in vivo analgesic activity (Hot plate method) were determined by the well-known test protocol available in the literature. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids ect. The total phenolics content of hydroalcoholic leaves extract was (1.458mg/100mg), followed by flavonoids (1.025mg/100mg). The results of this study showed the evidence that the hydroalcoholic leaves extracts of C. bonducella have considerable antioxidant potential, in vitro enzyme inhibitory and analgesic activity. The results of this study indicate that the hydroalcoholic extract of C. bonducella leaves has significant pharmacological properties that may lead to new drug development.
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